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oai:www.repository.cam.ac.uk:1810/3651772025-01-09T20:06:59Zcom_1810_245928com_1810_34581col_1810_273762

A Single Cell Approach to TCR Signal Strength https://doi.org/10.17863/CAM.106571 Ma, Claire 0000000242447535 Griffiths, Gillian Cytotoxic T lymphocytes (CTLs) play a key role in the cell-mediated immune response against virally-infected and tumourigenic cells, killing their targets through the release of cytolytic granules. T cell receptor (TCR) recognition of foreign peptides presented by Class I MHC molecules stimulates naïve CD8+ T cell differentiation to effector cells and triggers the cytolytic activity of effector CTLs. Signal transduction downstream of the TCR is a highly diverse and coordinated network of post-translational protein modifications that ultimately drive transcriptional, translational, metabolic and cytoskeletal changes in the cell. How cytotoxic T cells coordinate their molecular machinery in response to strong versus weak stimuli remains unclear. Utilising single-cell methods including mass cytometry and single-cell RNA-sequencing, this study demonstrates how naïve and restimulated cytotoxic T cells coordinate their responses against peptides of differing stimulation strengths. University of Cambridge 2024-01-01 Thesis Doctoral Doctor of Philosophy (PhD) eng https://www.repository.cam.ac.uk/handle/1810/365177 https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/f9954baa-dff3-438d-9095-6871deb0a5bb/download 87eda9de84448d1f82354d60eee3eb5f https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/e9d2141e-de1d-41c6-91a2-1397e2f8f50b/download 24a950f9e110f8bd770a132e7855f94f http://purl.org/NET/rdflicense/allrightsreserved CTL Cytof Cytotoxic T cells mass cytometry signal strength signalling single cell TCR