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Extracellular Interaction Screening Using CRISPR Activation 10.17863/CAM.39124 Chong, Zheng Shan Wright, Gavin Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors for high-affinity antibodies and low-affinity ligands can be unambiguously identified when used in pools or as individual binding probes. We apply this technique to identify ligands for the adhesion G-protein-coupled receptors and show that the Nogo myelin-associated inhibitory proteins are ligands for ADGRB1. This method will enable extracellular receptor-ligand identification on a genome-wide scale. University of Cambridge 2019-11-30 Thesis Doctoral Doctor of Philosophy (PhD) en https://www.repository.cam.ac.uk/handle/1810/291972 https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/b1d0fcca-fb14-4e91-81ae-10c11b8a4503/download 60e3c54dad1b1119996275dd48c6f2ce https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/ee4d27e8-2d69-4ee2-92ac-fff451fb60bc/download 87eda9de84448d1f82354d60eee3eb5f https://www.rioxx.net/licenses/all-rights-reserved/ CRISPR protein-protein interactions high-throughput screening