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DNA Origami Structures for Artificial Light-Harvesting and Optical Voltage Sensing 10.17863/CAM.21085 Hemmig, Elisa Alina Keyser, Ulrich F. In the past decade, DNA origami self-assembly has been widely applied for creating customised nanostructures with base-pair precision. In this technique, the unique chemical addressability of DNA can be harnessed to create programmable architectures, using components ranging from dye or protein molecules to metallic nanoparticles. In this thesis, we apply DNA nanotechnology for developing novel light-harvesting and optical voltage sensing nano-devices. We use the programmable positioning of dye molecules on a DNA origami plate as a mimic of a light-harvesting antenna complex required for photosynthesis. Such a structure allows us to systematically analyse optimal design concepts using different dye arrangements. Complementary to this, we use the resistive-pulse sensing technique in a range of electrolytes to characterise the mechanical responses of DNA origami structures to the electric field applied. Based on this knowledge, we assemble voltage responsive DNA origami structures labelled with a FRET pair. These undergo controlled structural changes upon application of an electric field that can be detected through a change in FRET efficiency. Such a DNA-based device could ultimately be used as a sensitive voltage sensor for live-cell imaging of transmembrane potentials. University of Cambridge 2018-05-19 Thesis Doctoral Doctor of Philosophy (PhD) en https://www.repository.cam.ac.uk/handle/1810/274005 controlled.access https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/5360ac37-6a7a-4ca2-aab6-bb46184134e0/download 87eda9de84448d1f82354d60eee3eb5f https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/98ff13c7-6a32-4ec4-83af-5e770f5591f9/download 711eec9425dcfadb55e342507f154b2f https://www.rioxx.net/licenses/all-rights-reserved/ DNA nanotechnology DNA origami single-molecule FRET nanocapillary nanopore artificial light-harvesting self-assembly optical voltage measurements