2026-09-21T15:29:44Zhttps://api.repository.cam.ac.uk/server/oai/requestoai:www.repository.cam.ac.uk:1810/2451242024-06-26T13:46:36Zcom_1810_195217com_1810_256065col_1810_219484
Development of micro analytical devices
10.17863/CAM.16075
Deshpande, Abhishek Girish
This thesis describes the design and development
of novel micro analytical devices for application
in on-line process analytics. The work describes the design, development, numerical simulation and
application of these devices for two specific cases: (i) electrochemical detection of bio(chemical)
species at micro-scale and (ii) separation and purification of biological reagents using immobilised
metal affinity chromatography at micro-litre scale.
Chapter 1 provides a general overview and background to the field of
process analytics, microreactors and theory related to the mass transfer inside the electrochemical microfluidic
devices and meso-chromatography columns. Chapter
2 provides an overview of microfabrication
methods and the numerical simulations employed for the development of micro analytical devices
used in this thesis.
Chapter 3 describes an experimental voltammetric study of enzyme cofactors in batch and
hydrodynamic systems and also provides a numerical investigation of mass transfer over electrodes
inside microreactors. Chapter 4 investigates the effect of
hydrodynamic focusing within a
microfluidic device in detail, using experimental and numerical techniques. The quantification of
the results was carried out using a pseudo two-dimensional, steady
state backward implicit finite difference model. A series of studies, interrogating
the effects of volumetric flow rate, volume ratio
and lead-in length, were carried
out to quantitatively investigate hydrodynamic focusing.
Chapter 5 details the development and fabrication of patterned photopolymerised and
electrochemically polymerised (conducting) monoliths with dimensions in the range of
100-1000μm. The photopolymerised monoliths were characterised using hydrodynamic methods in
order to study the flow profile. Electrochemical techniques were used to characterise the
conducting monoliths and its composites, using N,N,N’,N’-tetramethyl-p-phenylenediamine.
Chapter 6 describes an application of the
photopatterned monoliths. A meso-chromatography
column was fabricated and immobilised metal affinity chromatography at meso and micro-litre
scale was studied inside these columns. Proteins with polyhistidine tags were shown to be
successfully separated, purified and quantified under batch and hydrodynamic conditions.
University of Cambridge
2009-11-17
Thesis
Doctoral
Doctor of Philosophy (PhD)
en
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